A Plant Protease For Protein Hydrolysis

Description

DEERLAND BROMELAIN is a proteolytic enzyme (E.C.3.4.22.4) isolated from the pineapple stem.  The broad substrate specificity of DEERLAND BROMELAIN enables the enzyme to efficiently hydrolyze most soluble proteins.  Casein, hemoglobin, gelatin, soya, fish and other proteins are hydrolyzed to lower molecular weight peptides and amino acids which are soluble in trichloroascetic acid.  Although amino acids are liberated during hydrolysis, the reaction does not necessarily proceed to completion.

 

Product                                                Activity                     Comment

DEERLAND BROMELAIN                NLT 850 BTU/g          Unstandardized Concentrate

 

Properties

Form       Amorphous dry powders

Color      Tan to light brown

Odor       Free of offensive odor

Taste       Free of offensive taste

 

Inactivation

The catalytic activity of DEERLAND BROMELAIN depends on the presence of a sulfhydryl group at the active site.  Oxidizing agents, agents, and heavy metal inhibit the enzyme by binding to the thiol group.  Several metal ions including copper, mercury, lead, zinc, and iron inhibit DEERLAND BROMELAIN.  The inhibitory effect can be reversed by the addition of L-cysteine.

 

Use Level

Enzyme requirements are generally dictated by processing conditions.  They will vary with substrate concentration, degree of desired protein hydrolysis, pH, temperature, and time of hydrolysis.  In general, increasing the DEERLAND BROMELAIN concentration will increase the rate of protein hydrolysis and shorten the time necessary for hydrolysis.

 

Storage Stability

In containers, under cool, dry conditions, the loss of activity is normally less than 10% over one year.  Storage life can be extended by storing under refrigeration at 5°C.

 

Packaging

Deerland Bromelain is available in 40 Kg fiber drums with ring seal and lined with a polyethylene bag.  Customizing is available.